REAL-TIME PCR DETECTION SYSTEM
CFX96 Touch
The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions.
Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch System is designed to advance your qPCR.
Key Features and Benefits
With the CFX96 Touch Real-Time PCR Detection System you can:
- Set up your system quickly — easy installation and factory-calibrated optics
- Minimize sample and reagent use — up to 5-target multiplexing with sample volumes as low as 10 µl
- Optimize reactions in a single run — thermal gradient feature
- Analyze data faster — visualize all run data at once and export only the data you need in the format you want
- Use advanced data analysis tools — normalized gene expression using CFX Maestro Software
- Configure the system to fit your needs — run without a computer, run up to 4 systems from 1 computer, or integrate with the CFX Automation System II for higher throughput
- Combine the CFX96 Touch System with good laboratory practice standards — use CFX Maestro Software, Security Edition for data collection and analysis to simplify compliance with U.S. FDA 21 CFR Part 11 regulations
CFX Maestro Software Works Seamlessly With PrimePCR Assays And Panels
The CFX Automation System II works with all CFX Real-Time PCR Detection Systems to enable walk-away, high-throughput qPCR operation. Integrate this robotic plate handler with one or two CFX Systems to maximize throughput while maintaining a compact footprint.CFX Maestro Software Works Seamlessly With PrimePCR Assays And Panels
The expert design and wet-lab validation of all PrimePCR Assays ensure optimal assay performance. The integration of CFX Maestro Software with PrimePCR Assays aids in the analysis and interpretation of experimental results.- Begin analyzing data in a single step by importing target, reference, and control information from a PrimePCR run file directly into CFX Maestro Software
- Combine data from multiple plates into a Gene Study to rapidly screen large numbers of targets or samples
- Use powerful data visualization tools, such as hierarchical clustering and color-matched expression levels, to identify individual targets or clusters to consider for further investigation
Specifications
| Thermal Cycler | |
|---|---|
| Chassis | C1000 Touch™ |
| Maximum ramp rate, °C/sec | 5 |
| Average ramp rate, °C/sec | 3.3 |
| Heating and cooling method | Peltier |
| Lid, °C | Heats up to 105 |
| Temperature | |
| Range, °C | 0–100 |
| Accuracy, °C | ±0.2 of programmed target at 90°C |
| Uniformity, °C | ±0.4 well-to-well within 10 sec of arrival at 90°C |
| Gradient | |
| Operational range, °C | 30–100 |
| Programmable span, °C | 1–24 |
| Optical Detection | |
| Excitation | 6 filtered LEDs |
| Detection | 6 filtered photodiodes |
| Range of excitation/emission wavelengths, nm | 450–730 |
| Sensitivity | Detects 1 copy of target sequence in human genomic DNA |
| Dynamic range | 10 orders of magnitude |
| Scan Time | |
| All channels, sec | 12 |
| FAM/SYBR® Green only, sec | 3 |
| Software | |
| Operating systems | Windows 7, Windows 8, Windows 10 |
| Multiplex analysis | Up to 5 targets per well |
| System | |
| Licensed for real-time PCR | Yes |
| Sample capacity, wells | 96 |
| Sample size, µl | 1–50 (10–25 recommended) |
| Communication interface | USB 2.0 |
| Electrical approvals | IEC, CE |
| Dimensions (W x D x H), cm/in | 33 x 46 x 36 /13 x 18 x 14 |
| Weight, kg/lb | 21/47 |